Back to MASTER LAB page

SEE MRO LAB and crab joint receptor labs for overview movies and protocols

Published protocol (text and movie crab leg PD) : http://www.jove.com/video/51050/proprioception-tension-receptors-crab-limbs-student-laboratory

Published protocol (text and movie crayfish MRO) :
http://www.jove.com/video/2323/muscle-receptor-organs-crayfish-abdomen-student-laboratory-exercise

A start on the two posters: (Sample from past years)

Papers from past Bio446/650 classes

Dayaram,V., Malloy, C., Martha, S., Alvarez, B., Chukwudolue, I., Dabbain, N., D.mahmood, D., Goleva, S., Hickey, T., Ho, A., King, M., Kington, P., Mattingly, M., Potter, S., Simpson, L., Spence, A., Uradu, H., Van Doorn, J.L., and Cooper, R.L. (2017). Stretch activated channels in proprioceptive chordotonal organs of crab and crayfish are sensitive to Gd3+ but not amiloride, ruthenium red or low pH. IMPLUSE The Premier Undergraduate Neuroscience Journal. [PDF] https://impulse.appstate.edu/issues/2017

Malloy, C., Dayaram,V., Martha, S., Alvarez, B., Chukwudolue, I., Dabbain, N., D.mahmood, D., Goleva, S., Hickey, T., Ho, A., King, M., Kington, P., Mattingly, M., Potter, S., Simpson, L., Spence, A., Uradu, H., Van Doorn, J.L., Weineck, K. and Cooper, R.L. (2017). The effects of neighboring muscle injury on proprioception responses in crayfish and crab. J. of Exp. Zoology. 327(6):366–379. [PDF]

Dayaram,V., Malloy, C., Martha, S., Alvarez, B., Chukwudolue, I., Dabbain, N., D.mahmood, D., Goleva, S., Hickey, T., Ho, A., King, M., Kington, P., Mattingly, M., Potter, S., Simpson, L., Spence, A., Uradu, H., Van Doorn, J.L., and Cooper, R.L. (2017). The effect of CO2, intracellular pH and extracellular pH on mechanosensory proprioceptor responses in crayfish and crab. American Journal of Undergraduate Research 14(3):85-99 [PDF].

Wycoff, S., Weineck, K., Conlin, S., Grau, E., Bradley, A., Cantrell, D., Eversole, S., Grachen, C., Hall, K., Hawthorne, D., Kinmon, C., Ortiz Guerrero, P., Patel, B., Samuels, K., Suryadevara, C., Valdes, G., Ray, A., Fleckenstein, L., Piana, E., Cooper, R.L. (2018) Investigating potential effects of clove oil (eugenol) in model crustaceans. IMPLUSE pp. 1-21 https://impulse.appstate.edu/articles/2018/effects-clove-oil-eugenol-proprioceptive-neurons-heart-rate-and-behavior-model-crustac

Saelinger, C.M., McNabb, M.C., Trent, S., Danley, M. and Cooper, R.L. (2019). Effects of eugenol on nerve conduction and synaptic transmission at neuromuscular junction in an amphibian. IMPULSE https://impulse.appstate.edu/articles/2019/investigating-effects-eugenol-nerve-conduction-and-synaptic-transmission-neuromuscular

Stanback, M., Stanback, A.E., Akhtar, S., Basham, R., Chithrala, B., Collis, B., Heberle, B.A., Higgins, E., Lane, A., Marella, S., Ponder, M., Raichur, P., Silverstein, A., Stanley, C., Vela, K. and Cooper, R.L. (2019). The effect of lipopolysaccharides on primary sensory neurons in crustacean models. IMPLUSE https://impulse.appstate.edu/articles/2019/effect-lipopolysaccharides-primary-sensory-neurons-crustacean-models

Stanley, C.E., Adams, R., Nadolski, J., Amrit, E., Barrett, M., Bohnett, C., Campbell, K., Deweese, K., Dhar, S., Gillis, B., Hill, C., Inks, M., Kozak, K., Larson, A., Murtaza, I., Nichols, D., Roberts, R., Tyger, H., Waterbury, C. and Cooper, R.L. (2020). The effects of tricaine mesylate on arthropods: Crayfish, crab and Drosophila. Invertebrate Neuroscience. 20, Article number: 10 (2020) https://doi.org/10.1007/s10158-020-00243-5 [PDF] [Corrected graphical abstract-dowload Tiff file]

Nethery, B., Abou El-Ezz, M., Brown, C., Calderaro, T., Evans, C., Grant, T., Hazelett, R., High, C., Buendia Castillo, D., Ilagan, T., Klier, J., Marguerite, N., Marino, F., McCubbin, S., Meredith, N., Naidugari, P., Russell, W., Sommers, N., and Cooper, R.L. (2021) The effects of Riluzole on sensory and motor nerve function. IMPULSE. This was a Bio446 class project for Fall 2020. https://impulse.appstate.edu/articles/2021/effects-riluzole-sensory-and-motor-nerve-function [PDF]

McCubbin, S., Abou El-Ezz, M., Brown, C., Calderaro, T., Evans, C., Grant, T., Hazelett, R., High, C., Buendia Castillo, D., Ilagan, T., Klier, J., Marguerite, N., Marino, F., Meredith, N., Naidugari, P., Nethery, B., Russell, W., Sommers, N., and Cooper, R.L. (2021) The effects of Levetiracetam on glutamatergic synaptic transmission IMPULSE. This was a Bio446 class project for Fall 2020.https://impulse.appstate.edu/articles/2021/effects-levetiracetam-glutamatergic-synaptic-transmission [PDF]

Over all PDFs related to projects

Rydquistetal2007MRO-crayfish

PPT talk on PD organ

Molecular mechanisms 2011 of mechanotransduction

The sensation of stretch 2014

MRO-protocol MS word or PDF

Crab pd lab protocol MS word or PDF

Stretch activated review 2015

proprioception review 2012

review 2012 touch-Geffeney

Bewick and Banks 2015-Mammal muscle spindle

Biochemical Basis of Touch-molecular-review-2002

Signaling by Sensory Receptors

Mechanosensory molecules and circuits in C elegans-review

Pickpocket1 Is an Ionotropic Molecular Sensory Transducer

amiloride block in Drosophila neurons-2nd paper..... photo amiloride block in Drosophila MD neurons

Piezo Is Essential for Amiloride-Sensitive2015

Cooper, R.L. and Govind, C.K. (1991) Axon composition of the proprioceptive PD nerve during growth and regeneration of lobster claws. Journal of Experimental Zoology 260:181-193.[Full PDF]

Hartman, H.B. and Cooper, R.L. (1994) Regeneration and molting effects on a proprioceptor organ in the Dungeness crab, Cancer magister. Journal of Neurobiology 25:461-471[Full PDF]

Cooper, R.L. (2008) Mapping proprioceptive neurons on chordotonal organs in the crab, Cancer magister Crustaceana 81:447-475 [PDF]

(also how to move leg ... movie by Maddie Stanback) (Here....MPEG or window media)

Movies:

TBA-?